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• 研究论文 •    下一篇

浅黄恩蚜小蜂寄生对烟粉虱海藻糖含量、酶活和热激蛋白表达水平的影响

王颖颖1,2, 王甦2, 张帆2, 李飞1   

  1. 1. 南京农业大学植物保护学院, 南京 210095;2. 北京市农林科学院植物保护环境保护研究所, 北京 100097
  • 收稿日期:2015-03-10 修回日期:1900-01-01 出版日期:2015-06-08 发布日期:2015-06-08
  • 通讯作者: 王甦, 李飞

The Effect of Parasitization by Encarsia sophia on the Content of Trehalose, the Activity of Trehalase and Expression of Heat Shock Protein Genes of Bemisia tabaci Nymph

WANG Yingying1,2, WANG Su2, ZHANG Fan2, LI Fei1   

  1. 1. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;2. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
  • Received:2015-03-10 Revised:1900-01-01 Online:2015-06-08 Published:2015-06-08

摘要: 本文以烟粉虱Bemisia tabaci Gennadius及其优势寄生蜂浅黄恩蚜小蜂Encarsia sophia(Girault & Dodd)为研究对象,测定了浅黄恩蚜小蜂寄生烟粉虱若虫后6、12、24、48和72 h,寄主体内海藻糖含量、海藻糖酶活性变化以及3种热激蛋白基因的即时表达情况.结果表明,自寄生蜂寄生后烟粉虱体内海藻糖含量先升高后降低,而海藻糖酶活性则先降低后升高,二者的变化拐点均表现在寄生后24 h.此外,寄生能够诱导烟粉虱hsp90过量表达,但同时抑制hsp70的表达.热激蛋白hsp20的表达量先升高后降低,且3种基因均在寄生后12 h开始出现表达差异.本研究证明海藻糖和热激蛋白参与了寄生蜂与寄主互作体系,同时探讨了二者在寄生蜂与寄主互作过程中的具体作用,对于揭示寄生蜂-寄主互作机制有一定的参考价值.

Abstract: Changes of trehalose and trehalase activity and expression levels of three heat shock protein genes in Bemisia tabaci were measured at 6, 12, 24, 48 and 72 h after parasitization by Encarsia sophia. Contents of trehalose peaked at 24 h and then decreased, while trehalase activity was low until 24 h after parasitization and then increased. Parasitization induced high expression of hsp90 gene but suppressed expression of hsp70 gene. Expression level of hsp20 gene increased in the early stage after parasitization and was then reduced. The three genes were differentially expressed starting at 12 h after parasitization. This study indicates the involvement of trehalose and heat shock protein in parasitoid-host immunity interaction.

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